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Download COA here: PNC-27_COA.pdf
Please note: if you have a different Batch ID please contact us for the latest COA.

PNC-27

Synonyms / Designations: PNC-27, p53-derived anticancer peptide, HDM-2–binding peptide
CAS Number: 1159861-00-3
Molecular Formula: C188H293N53O44S
Molecular Weight: 4031.72 g/mol
Peptide Classification: Synthetic p53-derived peptide; amphipathic anticancer research peptide
Purity: ≥ 99 % (HPLC, typical)
Appearance: White to off-white lyophilised powder
Pack Size: 5 mg (total)
Storage: Desiccated, protected from light, stored at –20 °C
Solubility: Soluble in sterile water and buffered aqueous solutions

Description & Mechanism

PNC-27 is a synthetic peptide derived from the HDM-2–binding domain of the tumor suppressor protein p53, conjugated to a membrane-penetrating leader sequence. It was developed as a structurally defined research tool to investigate selective cancer cell membrane disruption and p53-associated anticancer mechanisms.

In biochemical and cellular research models, PNC-27 has been shown to preferentially interact with HDM-2 proteins expressed on the surface of certain cancer cells, leading to membrane pore formation and rapid necrotic cell death. Experimental studies focus on peptide–membrane interactions, cancer cell selectivity, and downstream signalling effects under controlled conditions. Observed effects depend on experimental design, concentration, and model system.

Applications in Research

• Investigation of p53-related tumour suppressor mechanisms
• Studies of cancer cell–selective membrane disruption
• In vitro cytotoxicity and membrane interaction assays
• Use as a reference compound in peptide-based anticancer research

Handling, Reconstitution & Stability

• Weigh under dry conditions; peptide is hygroscopic
• Reconstitute in sterile water or suitable buffered aqueous solution
• Avoid vigorous agitation during dissolution
• Filter sterilize if required (0.22 µm) prior to use
• Aliquot and store reconstituted solutions at –20 °C or lower
• Avoid repeated freeze–thaw cycles

Specifications Summary

Purity: ≥ 99 %
Appearance: White to off-white lyophilised powder
Peptide Type: Synthetic p53-derived peptide
Molecular Weight: 4031.72 g/mol
Solubility: Water, buffered aqueous solutions
Storage: –20 °C, desiccated, protected from light
Pack Size: 5 mg

Precautions & Notes

• Experimental behaviour of PNC-27 is influenced by membrane composition, peptide concentration, and model system
• Peptide exhibits selective cytotoxicity in cancer cell models and should be handled with care
• Not intended to infer clinical safety or therapeutic use
• Appropriate controls are essential in cytotoxicity studies
• Intended strictly for laboratory research use; not for human or veterinary application

References

Kanovsky M. et al. Selective cancer cell membrane disruption by p53-derived peptide PNC-27. Proceedings of the National Academy of Sciences, 2001.
https://www.pnas.org/doi/10.1073/pnas.211280698

Makis W. et al. Mechanisms of action of p53-derived peptides in cancer research. Cancer Letters, 2003.
https://pubmed.ncbi.nlm.nih.gov/20080680/

Vogelstein B., Lane D., Levine A.J. Surfing the p53 network. Nature, 2000.
https://pubmed.ncbi.nlm.nih.gov/11099028/

Keywords: PNC-27, p53 Peptide, Anticancer Peptide, HDM-2 Binding Peptide, Synthetic Research Peptide