• OP Labs formerly Oxford Peptides
• Batch HPLC tested at ≥ 99% purity
• Storage: –20 °C long term; 2–8 °C short term
• Sold for research purposes only

Bronchogen

Synonyms / Designations: Bronchogen; AEDL
Amino Acid Sequence: Ala-Glu-Asp-Leu (AEDL)
CAS Number: N/A
Molecular Formula: C18H30N4O9
Molecular Weight: 446.45 g/mol
Purity: ≥ 99% (HPLC)
Appearance: White to off-white lyophilised powder
Pack Size: 20 mg (total)
Storage: Desiccated, protected from light, stored at –20 °C or below
Solubility: Soluble in water and buffered aqueous solutions

Description & Mechanism

Bronchogen is a short synthetic tetrapeptide used as a molecular probe for studying tissue-specific regulation of gene expression in bronchial-epithelium model systems. In cultured human bronchial epithelial cells it has been reported to modulate transcription of mucin and surfactant-associated genes (MUC4, MUC5AC, SFTPA1) and to alter levels of proliferation- and apoptosis-associated proteins.

Peptides of this class are studied as candidate cell-penetrating regulators that may interact with DNA and chromatin to influence promoter activity. Bronchogen is therefore used to examine putative peptide–DNA interactions and downstream transcriptional readouts in vitro; observed effects depend on concentration, model system and exposure conditions.

Applications in Research

• In-vitro modulation of gene/protein expression in human bronchial epithelial cell cultures (MUC4, MUC5AC, SFTPA1, Ki-67, p53)
• Cellular models of epithelial proliferation and apoptosis
• Probe compound for short-peptide / DNA-promoter interaction studies

Specifications Summary

Precautions & Notes

• Experimental behaviour is dependent on concentration, model system and exposure conditions
• Appropriate experimental controls are recommended
• Prolonged exposure to light, elevated temperature or aqueous environments may reduce stability
• Intended strictly for laboratory research use; not for human or veterinary application

References

Khavinson VKh, et al. Peptide regulation of gene expression and protein synthesis in bronchial epithelium. Lung. 2014;192(5):781-791.
https://pubmed.ncbi.nlm.nih.gov/25015171/

Monaselidze JR, Khavinson VKh, et al. Effect of the peptide bronchogen (Ala-Asp-Glu-Leu) on DNA thermostability. Bull Exp Biol Med. 2011;150(3):375-377.
https://pubmed.ncbi.nlm.nih.gov/21240358/

Khavinson VKh, Popovich IG, et al. Peptide Regulation of Gene Expression: A Systematic Review. Molecules. 2021;26(22):7053.
https://www.mdpi.com/1420-3049/26/22/7053

Keywords: Bronchogen, AEDL, synthetic tetrapeptide